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crop seq vector  (Addgene inc)


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    Structured Review

    Addgene inc crop seq vector
    Crop Seq Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/crop seq vector/product/Addgene inc
    Average 95 stars, based on 38 article reviews
    crop seq vector - by Bioz Stars, 2026-04
    95/100 stars

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    Journal: iScience

    Article Title: Generation and characterization of inducible KRAB-dCas9 iPSCs from primates for cross-species CRISPRi

    doi: 10.1016/j.isci.2024.110090

    Figure Lengend Snippet:

    Article Snippet: Briefly, gRNAs were cloned into the BsmBI-digested (New England BioLabs, R0739S) CROP-seq-opti vector by Gibson Assembly using the NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs, E2621L) and NEB Stable Competent E. coli (New England BioLabs, C3040I) were transformed with the assembled plasmids.

    Techniques: Virus, Recombinant, Membrane, Lysis, CRISPR, DNA Extraction, Western Blot, Transfection, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Stripping, Software, Microscopy